3C0L
UVDE K229R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-16 |
| Detector | MAR225 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 107.110, 107.110, 91.120 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.180 - 3.150 |
| R-factor | 0.19703 |
| Rwork | 0.195 |
| R-free | 0.24940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bzg |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.945 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.180 | 3.320 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.134 | 0.366 |
| Number of reflections | 5367 | |
| <I/σ(I)> | 18.3 | 6.6 |
| Completeness [%] | 95.3 | 95.3 |
| Redundancy | 10.7 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 295 | 0.1M Acetate, pH5.6, 1M Naformate, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
