3C0H
CASK CaM-Kinase Domain- AMPPNP complex, P1 form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-29 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.387, 59.675, 61.086 |
| Unit cell angles | 90.00, 105.57, 105.63 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.19058 |
| Rwork | 0.188 |
| R-free | 0.23862 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | rat CamKI |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.137 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 30806 | |
| <I/σ(I)> | 14.5 | 1.4 |
| Completeness [%] | 96.9 | 87 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 12.5%(v/v) ethylene glycol, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
