3C0G
CASK CaM-Kinase Domain- 3'-AMP complex, P1 form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-29 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.328, 60.267, 60.553 |
| Unit cell angles | 90.00, 106.49, 105.80 |
Refinement procedure
| Resolution | 20.000 - 2.190 |
| R-factor | 0.21074 |
| Rwork | 0.208 |
| R-free | 0.26969 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | rat CamKI |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.145 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.300 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Number of reflections | 32667 | |
| <I/σ(I)> | 12.4 | 1.5 |
| Completeness [%] | 89.8 | 76.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 12.5%(v/v) ethylene glycol, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
