3C08
Crystal structure the Fab fragment of matuzumab/EMD72000 (Fab72000)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-07 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.821, 61.377, 102.675 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.150 |
| R-factor | 0.226 |
| Rwork | 0.224 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB id 1L7I |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.329 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.700 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.102 | 0.417 |
| Number of reflections | 20191 | |
| <I/σ(I)> | 7.2 | 3.618 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.3 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | 1.8M ammonium sulfate, 0.1M MES, pH 6.5, vapor diffusion, temperature 298K |
