3BZX

Crystal structure of the mutated H265A EscU C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-05-29
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	I 2 2 2
Unit cell lengths	55.739, 59.024, 72.132
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.000 - 1.600
R-factor	0.197
Rwork	0.196
R-free	0.21900
RMSD bond length	0.015
RMSD bond angle	1.537
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.690	16.000	1.660
High resolution limit [Å]	1.600	3.440	1.600
Rmerge	0.034	0.032	0.237
Number of reflections	15546
<I/σ(I)>	23
Completeness [%]	96.8	90.7	99.6
Redundancy	6.5	6.3	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	8	293	K2PO4 Peg3350, NaCl, Tris, pH 8.0, Microbatch, temperature 293K