3BZR
Crystal structure of EscU C-terminal domain with N262D mutation, Space group P 41 21 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 50.928, 50.928, 100.293 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 16.000 - 1.646 |
R-factor | 0.211 |
Rwork | 0.210 |
R-free | 0.24800 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.772 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.410 | 16.000 | 1.710 |
High resolution limit [Å] | 1.646 | 3.540 | 1.646 |
Rmerge | 0.044 | 0.030 | 0.824 |
Number of reflections | 16671 | ||
<I/σ(I)> | 14.8 | ||
Completeness [%] | 99.9 | 99.9 | 100 |
Redundancy | 14.1 | 14.3 | 12.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.5 | 293 | HEPES, Jeffamine ED-2001, NaCl, Tris, pH 6.5, Microbatch, temperature 293K |