3BZQ
High resolution crystal structure of Nitrogen Regulatory Protein (Rv2919c) of Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | H 3 |
| Unit cell lengths | 77.149, 77.149, 51.840 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.960 - 1.400 |
| R-factor | 0.2085 |
| Rwork | 0.208 |
| R-free | 0.22566 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gnk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.007 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.960 |
| High resolution limit [Å] | 1.400 |
| Number of reflections | 21338 |
| Completeness [%] | 99.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 0.1 M Immidazole, pH 6.5, 1.4 M Sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
