3BZK

Crystal Structure of the Tex protein from Pseudomonas aeruginosa, crystal form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-12-01
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	56.214, 106.688, 139.703
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.951 - 2.300
R-factor	0.249
Rwork	0.221
R-free	0.26600
Starting model (for MR)	3bzc
RMSD bond length	0.004
RMSD bond angle	0.605
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.380
High resolution limit [Å]	2.300	4.930	2.300
Rmerge	0.084	0.052	0.507
Number of reflections	38046
<I/σ(I)>	16.6
Completeness [%]	99.4	100	99.3
Redundancy	9	12.5	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	18% PEG 4000, 100mM Sodium Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K