3BZE

The human non-classical major histocompatibility complex molecule HLA-E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Spacegroup name	P 1 21 1
Unit cell lengths	104.778, 73.354, 131.535
Unit cell angles	90.00, 112.70, 90.00

Refinement procedure

Resolution	15.000 - 2.500
R-factor	0.2449
Rwork	0.242
R-free	0.29625
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mhe
RMSD bond length	0.025
RMSD bond angle	2.904
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Rmerge	0.052
Number of reflections	63040
<I/σ(I)>	21.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3	293	14-19% PEG 3350, 2% MPD, 0.2M MgCl2, 0.1M Tris, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K