3BZC
Crystal Structure of the Tex protein from Pseudomonas aeruginosa, crystal form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.165, 131.849, 144.017 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.281 - 2.270 |
| R-factor | 0.288 |
| Rwork | 0.240 |
| R-free | 0.27400 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.561 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.350 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.088 | 0.510 |
| Number of reflections | 41117 | |
| <I/σ(I)> | 11 | 2.5 |
| Completeness [%] | 79.4 | 35.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 286 | 19% PEG 3350, 0.1M Bis-Tris, 0.17 M ammonium sulfate, 10% glycerol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 286.0K |
