3BYW
Crystal structure of an extracellular domain of arabinofuranosyltransferase from Corynebacterium diphtheriae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.885, 80.619, 115.826 |
| Unit cell angles | 90.00, 110.91, 90.00 |
Refinement procedure
| Resolution | 35.250 - 2.350 |
| R-factor | 0.2117 |
| Rwork | 0.209 |
| R-free | 0.26315 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.713 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.250 | 2.400 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.074 | 0.400 |
| Number of reflections | 58325 | |
| <I/σ(I)> | 24.4 | 2.9 |
| Completeness [%] | 96.7 | 99.1 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 289 | 0.1M Acetate, 0.2M Zinc acetate, 10% PEG 3000, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
