3BYL

Crystal structure of B. subtilis levansucrase mutant E342A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-03-03
Detector	MAC Science DIP-2030
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	51.133, 67.281, 123.622
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.100
R-factor	0.182
Rwork	0.180
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1oyg
RMSD bond length	0.009
RMSD bond angle	1.207
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.140
High resolution limit [Å]	2.070	2.070
Rmerge	0.037	0.068
Number of reflections	26368
<I/σ(I)>	25	25.1
Completeness [%]	98.8	98.8
Redundancy	4.5	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	6.3	291	diammonium phosphate, sodium acetate, pH 6.3, MICRODIALYSIS, temperature 291K