3BYD
Crystal structure of beta-lactamase OXY-1-1 from Klebsiella oxytoca
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-09-19 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.0292 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.545, 73.431, 84.561 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.470 - 1.930 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bza |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.236 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.000 | 2.000 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.052 | 0.129 |
| Number of reflections | 21787 | |
| <I/σ(I)> | 14.8 | |
| Completeness [%] | 96.9 | 96.1 |
| Redundancy | 4.6 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 296 | 25%(w/v) PEG 4000, 0.1M sodium acetate buffer, 0.2M ammonium sulfate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
