3BY7

CRYSTAL STRUCTURE OF A PROTEIN STRUCTURALLY SIMILAR TO SM/LSM-LIKE RNA-BINDING PROTEINS (JCVI_PEP_1096686650277) FROM UNCULTURED MARINE ORGANISM AT 2.60 A RESOLUTION

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2007-08-19
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.95373, 0.97957, 0.97942
Spacegroup name	C 1 2 1
Unit cell lengths	108.250, 77.180, 71.470
Unit cell angles	90.00, 113.82, 90.00

Refinement procedure

Resolution	27.057 - 2.600
R-factor	0.238
Rwork	0.235
R-free	0.28500
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.247
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.057	27.057	2.690
High resolution limit [Å]	2.600	5.570	2.600
Rmerge	0.047	0.021	0.404
Number of reflections	16122	3047	2946
<I/σ(I)>	9.46	22.6	1.8
Completeness [%]	95.7	91.6	92.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	277	NANODROP, 0.2M Ca Acetate, 20.0% PEG 3350, No Buffer pH 7.3, VAPOR DIFFUSION, SITTING DROP, temperature 277K