3BXW
Crystal Structure of Stabilin-1 Interacting Chitinase-Like Protein, SI-CLP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-08 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9745 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 100.110, 100.110, 249.970 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| R-factor | 0.243 |
| Rwork | 0.222 |
| R-free | 0.25300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.711 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.127 | 0.751 |
| Total number of observations | 38872 | |
| Number of reflections | 40805 | |
| <I/σ(I)> | 5.5 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.4 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 2M(NH4)2SO4, 200mM NaCl, 100mM MgCl2, PH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
