3BXV
Crystal structure studies on sulfur oxygenase reductase from Acidianus tengchongensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 77 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-12-01 |
| Detector | RIGAKU |
| Wavelength(s) | 1 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 159.130, 159.130, 159.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.050 - 2.700 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cb2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.730 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.082 | 0.377 |
| Number of reflections | 9761 | |
| <I/σ(I)> | 22.7 | 3.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 303 | 1.08M NaCitr3, 0.1M Na-Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
