3BXQ
The structure of a mutant insulin uncouples receptor binding from protein allostery. An electrostatic block to the TR transition
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-21 |
| Wavelength(s) | 1.033 |
| Spacegroup name | H 3 |
| Unit cell lengths | 81.390, 81.390, 34.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.620 - 1.300 |
| Rwork | 0.206 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ins |
| RMSD bond angle | 0.170 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.620 | 1.380 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.040 | 0.132 |
| Number of reflections | 39411 | |
| <I/σ(I)> | 21.2 | 10 |
| Completeness [%] | 95.4 | 77.9 |
| Redundancy | 5.11 | 2.89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.02 M Tris, 0.05 M sodium citrate, 5% acetone, 0.03% phenol, 0.01% zinc acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
