3BXO
Crystal Structure of Streptomyces venezuelae DesVI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-18 |
| Detector | Bruker Platinum 135 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.170, 56.096, 116.482 |
| Unit cell angles | 90.00, 92.50, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.169 |
| Rwork | 0.165 |
| R-free | 0.25400 |
| Structure solution method | MAD, MR |
| Starting model (for MR) | Selenomethionine enriched DesVI model from MAD phasing |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.000 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHASER |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 36362 | |
| <I/σ(I)> | 9.4 | 2.6 |
| Completeness [%] | 89.8 | 76.5 |
| Redundancy | 2.7 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | PEG 5000 monomethyl ether, NaCl, S-adenosylmethionine, UDP-benzene, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
