3BXC

Monomeric Far-red Fluorescent Protein mKate Crystallized at pH 9.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	73.474, 104.481, 123.226
Unit cell angles	90.00, 106.09, 90.00

Refinement procedure

Resolution	29.710 - 2.600
Rwork	0.235
R-free	0.28200
RMSD bond length	0.008
RMSD bond angle	0.870
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge		0.445
Number of reflections	49319
Completeness [%]	89.2	65.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		9	293	20% w/v PEG 6000, 1.0M LiCl, 0.1M Bicine pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K