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3BXB

Monomeric Far-red Fluorescent Protein mKate Crystallized at pH 7.0

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 22-ID
Synchrotron siteAPS
Beamline22-ID
Temperature [K]100
Detector technologyCCD
DetectorMARMOSAIC 300 mm CCD
Wavelength(s)1.0
Spacegroup nameP 1 21 1
Unit cell lengths74.176, 104.967, 123.028
Unit cell angles90.00, 105.81, 90.00
Refinement procedure
Resolution29.840 - 2.600
Rwork0.170
R-free0.26300
RMSD bond length0.008
RMSD bond angle0.935
Data reduction softwareHKL-2000
Data scaling softwareSCALEPACK
Phasing softwareMOLREP
Refinement softwarePHENIX
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.0002.690
High resolution limit [Å]2.6002.600
Rmerge0.506
Number of reflections55727
Completeness [%]99.999.4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1729320% w/v PEG 6000, 1.0M LiCl, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

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PDB entries from 2024-05-15

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