3BX9
Monomeric Far-red Fluorescent Protein mKate Crystallized at pH 2.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 67.852, 67.852, 413.030 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uis |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.773 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.430 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.061 | 0.556 |
| Number of reflections | 52642 | |
| <I/σ(I)> | 5.63 | |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 14.1 | 14.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2 | 293 | 20% w/v PEG 3350, 0.2M ammonium citrate tribasic, 0.4M citric acid, pH 2.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
