3BWZ
Crystal structure of the type II cohesin module from the cellulosome of Acetivibrio cellulolyticus with an extended linker conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.455, 55.780, 87.912 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.090 - 1.200 |
| R-factor | 0.12352 |
| Rwork | 0.122 |
| R-free | 0.15209 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zv9 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.590 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.090 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.046 | 0.170 |
| Number of reflections | 53407 | |
| <I/σ(I)> | 13 | 4 |
| Completeness [%] | 95.8 | 99.1 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 2.0 M ammonium sulfate and 0.1 M sodium acetate trihydrate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
