3BWD
Crystal structure of the plant Rho protein ROP5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97634 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.420, 40.390, 57.180 |
| Unit cell angles | 90.00, 102.60, 90.00 |
Refinement procedure
| Resolution | 24.720 - 1.530 |
| R-factor | 0.24765 |
| Rwork | 0.247 |
| R-free | 0.26504 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ROP4-GDP of model with PDB ID 2NTY |
| RMSD bond length | 0.027 |
| RMSD bond angle | 1.957 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.600 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Number of reflections | 23836 | |
| Redundancy | 2.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 28%(w/v) PEG 3000, 100 mM MES pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
