3BUW
Crystal structure of c-Cbl-TKB domain complexed with its binding motif in Syk
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.793, 104.809, 52.778 |
| Unit cell angles | 90.00, 89.83, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.450 |
| Rwork | 0.224 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cbl |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.103 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 3.120 | 1.450 |
| Rmerge | 0.075 | 0.063 | 0.254 |
| Number of reflections | 113559 | ||
| <I/σ(I)> | 9 | ||
| Completeness [%] | 92.7 | 99.4 | 66 |
| Redundancy | 5.8 | 7.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.12M ammonium acetate, 18% PEG 3350, pH 6.5, vapor diffusion, hanging drop, temperature 298K |
