3BUS
Crystal Structure of RebM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2007-03-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9804 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 119.153, 119.153, 84.412 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.040 - 2.650 |
| R-factor | 0.217 |
| Rwork | 0.214 |
| R-free | 0.26000 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.330 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.740 |
| High resolution limit [Å] | 2.650 | 5.710 | 2.650 |
| Rmerge | 0.074 | 0.053 | 0.382 |
| Number of reflections | 17746 | ||
| <I/σ(I)> | 13.6 | ||
| Completeness [%] | 97.2 | 99.2 | 81.3 |
| Redundancy | 25.9 | 26.6 | 14.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | MEPEG 5000, NH4SO4, Na Acetate, pH 5.0, vapor diffusion, hanging drop, temperature 293K |
