3BUE
Crystal structure of the C-terminal domain hexamer of ArgR from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97848 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.219, 57.242, 57.328 |
| Unit cell angles | 66.19, 62.21, 82.00 |
Refinement procedure
| Resolution | 44.810 - 2.150 |
| R-factor | 0.1712 |
| Rwork | 0.168 |
| R-free | 0.23464 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b4b |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.822 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 28214 | |
| <I/σ(I)> | 11.77 | 2.26 |
| Completeness [%] | 95.0 | 84 |
| Redundancy | 1.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Drops containing 1 microliter protein solution (10 mg/mL) and 0.5 microliter reservoir solution equilibrated against the reservoir solution (20% PEG 10000, 0.1 M HEPES pH 7.5), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
