3BTP
Crystal structure of Agrobacterium tumefaciens VirE2 in complex with its chaperone VirE1: a novel fold and implications for DNA binding
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97900, 0.98000, 0.97600 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.020, 96.271, 112.483 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.140 - 2.300 |
| R-factor | 0.21178 |
| Rwork | 0.209 |
| R-free | 0.25576 |
| Structure solution method | SAD |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.260 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.109 | 0.386 |
| Number of reflections | 23980 | |
| <I/σ(I)> | 16.8 | 4.6 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 7.1 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH UNDER OIL | 7 | 292 | 20% PEG 6000, 0.2M NH4Cl, 1% Triton X-405, pH 7.0, MICROBATCH UNDER OIL, temperature 292K |
