3BTF
THE CRYSTAL STRUCTURES OF THE COMPLEXES BETWEEN BOVINE BETA-TRYPSIN AND TEN P1 VARIANTS OF BPTI.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM1A |
Synchrotron site | ESRF |
Beamline | BM1A |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1998-04 |
Detector | MARRESEARCH |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 75.630, 85.230, 122.870 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.800 |
Rwork | 0.194 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ptc |
RMSD bond length | 0.008 |
RMSD bond angle | 1.460 |
Data reduction software | DENZO |
Data scaling software | CCP4 |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.059 | 0.326 |
Number of reflections | 36655 | |
<I/σ(I)> | 8.4 | 2.2 |
Completeness [%] | 98.1 | 98.8 |
Redundancy | 4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | 37 * | 0.1 M HEPES PH 7.5 48% AMMONIUM SULPHATE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 27 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 48-50 (%sat) | |
3 | 1 | reservoir | HEPES | 0.1 (M) |