3BT8
Crystal Structure of Mutant Cyclophilin (R147A) from Leishmania donovani
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-09-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 65 |
| Unit cell lengths | 74.973, 74.973, 61.323 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 17.300 - 2.700 |
| Rwork | 0.158 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2haq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.050 | 0.152 |
| Number of reflections | 5450 | |
| <I/σ(I)> | 11 | 4.2 |
| Completeness [%] | 99.5 | 99.3 |
| Redundancy | 6.15 | 6.17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 0.01M TRIS, 10.0% PEG 3350, 0.02% AZIDE, PH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
