3BSD
Light harvesting protein from RC of Chlorobium tepidum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-12-28 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 167.695, 167.695, 167.695 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.820 - 2.300 |
| R-factor | 0.17838 |
| Rwork | 0.174 |
| R-free | 0.27328 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4bcl |
| RMSD bond length | 0.016 |
| RMSD bond angle | 3.309 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0013) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 118.680 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.068 | 0.640 |
| Number of reflections | 18207 | |
| <I/σ(I)> | 24.8 | 2.4 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 2.6M hexanediol, 0.2M magnesium chloride, 0.1M HEPES pH 7.5, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
