3BS7
Crystal structure of the Sterile Alpha Motif (SAM) domain of Hyphen/Aveugle
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9002 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 56.005, 88.470, 73.279 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.880 - 1.900 |
| R-factor | 0.217 |
| Rwork | 0.214 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.408 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.880 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 14692 | |
| Completeness [%] | 99.6 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1M Sodium citrate, 0.1M Cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
