3BS6
1.8 Angstrom crystal structure of the periplasmic domain of the membrane insertase YidC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-21 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.150, 55.640, 63.330 |
| Unit cell angles | 90.00, 101.12, 90.00 |
Refinement procedure
| Resolution | 38.900 - 1.800 |
| R-factor | 0.179 |
| Rwork | 0.178 |
| R-free | 0.21300 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.544 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.3.0008) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.990 | 1.890 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.067 | 0.428 |
| Number of reflections | 49763 | |
| <I/σ(I)> | 15.3 | 3.2 |
| Completeness [%] | 97.3 | 96.4 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 21% PEG3350, 0.2M calcium acetate, 10% ethylene glycol, VAPOR DIFFUSION, pH7.5, temperature 293K |
