3BRY
Crystal structure of the Ralstonia pickettii toluene transporter TbuX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-22 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9198 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 125.291, 125.244, 269.511 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.200 |
| R-factor | 0.268 |
| Rwork | 0.268 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.920 | 2.850 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Rmerge | 0.076 | 0.552 |
| Number of reflections | 27405 | |
| <I/σ(I)> | 2.1 | |
| Completeness [%] | 100.0 | 99.5 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 295 | 20% PEG 4000, 0.1 M magnesium acetate, 50 mM sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
