3BRX
Crystal Structure of calcium-bound cotton annexin Gh1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-14 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 132.663, 132.663, 61.338 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.440 - 2.500 |
| R-factor | 0.196 |
| Rwork | 0.193 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.641 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 114.890 | 66.370 | 2.640 |
| High resolution limit [Å] | 2.500 | 7.910 | 2.500 |
| Rmerge | 0.117 | 0.028 | 0.397 |
| Total number of observations | 3795 | 17291 | |
| Number of reflections | 21804 | ||
| <I/σ(I)> | 5.8 | 16.9 | 1.8 |
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 5.5 | 5 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | KH2PO4/Na2HPO4, CaCl2, pH 6.0, vapor diffusion, hanging drop, temperature 289K |
