3BRK
Crystal Structure of ADP-Glucose Pyrophosphorylase from Agrobacterium tumefaciens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-15 |
Wavelength(s) | 0.979 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 85.380, 93.793, 140.293 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.900 - 2.100 |
R-factor | 0.225 |
Rwork | 0.221 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.487 |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 79.000 |
High resolution limit [Å] | 2.100 |
Number of reflections | 33214 |
Completeness [%] | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 298 | 1.5M lithium sulfate, pH 7.5, vapor diffusion, temperature 298K |