3BRJ
Crystal structure of mannitol operon repressor (MtlR) from Vibrio parahaemolyticus RIMD 2210633
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97927, 0.97941 |
| Spacegroup name | P 41 |
| Unit cell lengths | 63.281, 63.281, 227.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.560 - 2.750 |
| R-factor | 0.21007 |
| Rwork | 0.207 |
| R-free | 0.27272 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.926 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.560 | 2.830 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.100 | 0.642 |
| Number of reflections | 23045 | |
| <I/σ(I)> | 17.5 | 1.5 |
| Completeness [%] | 99.2 | 91.6 |
| Redundancy | 4.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.2M tri-Ammonium citrate, 20% w/v PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
