3BRF
CSL (Lag-1) bound to DNA with Lin-12 RAM peptide, C2221
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-08 |
| Wavelength(s) | 1.0094 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 62.944, 95.972, 223.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.280 - 2.470 |
| R-factor | 0.232 |
| Rwork | 0.230 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.481 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0020) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.560 |
| High resolution limit [Å] | 2.470 | 5.320 | 2.470 |
| Rmerge | 0.070 | 0.052 | 0.549 |
| Number of reflections | 24758 | ||
| <I/σ(I)> | 11.8 | ||
| Completeness [%] | 99.2 | 98.3 | 95.4 |
| Redundancy | 5.9 | 6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | under oil microbatch | 6 | 277 | Bis-Tris, MgCl2, PEG 3350, Sorbitol, pH 6.0, under oil microbatch, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Bis | ||
| 10 | 1 | 2 | Sorbitol | ||
| 2 | 1 | 1 | Tris | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | PEG 3350 | ||
| 5 | 1 | 1 | Sorbitol | ||
| 6 | 1 | 2 | Bis | ||
| 7 | 1 | 2 | Tris | ||
| 8 | 1 | 2 | MgCl2 | ||
| 9 | 1 | 2 | PEG 3350 |
