3BRE
Crystal Structure of P.aeruginosa PA3702
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-18 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9771 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.510, 72.748, 106.095 |
| Unit cell angles | 90.00, 110.79, 90.00 |
Refinement procedure
| Resolution | 33.780 - 2.400 |
| Rwork | 0.242 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w25 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.480 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.066 | 0.459 |
| Number of reflections | 40110 | |
| <I/σ(I)> | 12.3 | 2 |
| Completeness [%] | 98.9 | 94.5 |
| Redundancy | 4.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 0.1 M Tris-Cl pH8.0, 2.9M NaCl, 15% xylitol, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
