3BRB
Crystal structure of catalytic domain of the proto-oncogene tyrosine-protein kinase MER in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-06 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.91790 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.385, 89.880, 69.537 |
| Unit cell angles | 90.00, 103.09, 90.00 |
Refinement procedure
| Resolution | 25.050 - 1.900 |
| R-factor | 0.19469 |
| Rwork | 0.192 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p0c |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.511 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.050 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 49802 | |
| <I/σ(I)> | 24.1 | 2.3929 |
| Completeness [%] | 98.3 | 88.7 |
| Redundancy | 7.2 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 290 | MER protein (38 mg/mL) was pre-incubated with 2.5 mM ATP and 10 mM MgCl2 for 3 hours at room temperature. Crystals were obtained at 290K against 29% PEG 400, 0.2M MgCl2, and 0.1 M Tris-HCl pH 8.5, VAPOR DIFFUSION, HANGING DROP |
