3BR8

Crystal structure of acylphosphatase from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Detector	MAR CCD 165 mm
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	26.857, 48.270, 59.567
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.790 - 1.330
R-factor	0.192
Rwork	0.190
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.271
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
High resolution limit [Å]	1.300
Number of reflections	66430

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION	5.5	277	50mM phosphate buffer, pH 5.5, LIQUID DIFFUSION, temperature 277K