3BQY
Crystal structure of a possible TetR family transcriptional regulator from Streptomyces coelicolor A3(2).
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-18 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97932, 0.97948 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 83.755, 83.755, 83.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.180 - 1.950 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.21000 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.280 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.990 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.103 | 0.539 |
| Number of reflections | 24079 | |
| <I/σ(I)> | 7.9 | 3.7 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 8.1 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 25% PEG 3350, 0.1M HEPES pH 7.5, 20% Sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
