3BQT
Crystal structure of a protein of unknown function from Listeria monocytogenes, tetragonal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-02 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.98000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 107.378, 107.378, 37.544 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.040 - 2.900 |
| R-factor | 0.269 |
| Rwork | 0.269 |
| R-free | 0.29900 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.770 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.120 | 0.230 |
| Number of reflections | 9166 | |
| <I/σ(I)> | 32 | 14.4 |
| Completeness [%] | 98.6 | 100 |
| Redundancy | 26.2 | 27.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.2M MgCl2, 0.1M Tris-HCl, 30% PEG 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
