3BQQ
Crystal Structure of Human Saposin D (triclinic)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-24 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.660, 41.120, 65.450 |
| Unit cell angles | 89.90, 81.20, 71.40 |
Refinement procedure
| Resolution | 64.550 - 2.000 |
| R-factor | 0.19756 |
| Rwork | 0.195 |
| R-free | 0.24217 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SAPOSIN D P212121 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.399 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.056 | 0.141 |
| Number of reflections | 24429 | |
| <I/σ(I)> | 20.4 | 6.73 |
| Completeness [%] | 92.6 | 91.2 |
| Redundancy | 3.52 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | PEG 4K, ZINC SULFATE, SODIUM ACETATE BUFFER, PH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
