3BQL
Structure of a chondroitin sulphate binding DBL3X domain from a var2csa encoded PfEMP1 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-12 |
| Detector | ADSC QUANTUM 1 |
| Wavelength(s) | 1.06 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.576, 86.990, 92.659 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.320 - 2.000 |
| R-factor | 0.22486 |
| Rwork | 0.222 |
| R-free | 0.27081 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.137 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.990 | 2.110 |
| High resolution limit [Å] | 1.800 | 2.000 |
| Rmerge | 0.083 | 0.348 |
| Number of reflections | 30158 | |
| <I/σ(I)> | 11.6 | 5.4 |
| Completeness [%] | 97.7 | 100 |
| Redundancy | 3.8 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 25% PEG 4K, 20mM HEPES pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
