3BQJ
VA387 polypeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-05-11 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.502 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 54.360, 96.800, 118.732 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 99.000 - 2.700 |
| Rwork | 0.227 |
| R-free | 0.26700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 99.000 | 99.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.960 | 2.300 |
| Rmerge | 0.071 | 0.032 | 0.234 |
| Number of reflections | 11228 | ||
| <I/σ(I)> | 12.1 | ||
| Completeness [%] | 97.3 | 91.6 | 87.9 |
| Redundancy | 3.5 | 3.8 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 22% peg 4k, 3% 5-diaminopentane, tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
