3BQ8

Crystal Structure of the E.coli PhoQ Sensor Domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	1998-06-15
Wavelength(s)	0.9879, 0.9793, 0.9790, 0.9641
Spacegroup name	P 1 21 1
Unit cell lengths	33.591, 113.965, 45.528
Unit cell angles	90.00, 109.45, 90.00

Refinement procedure

Resolution	19.170 - 2.500
R-factor	0.203
Rwork	0.203
R-free	0.30200
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.061	0.265
Number of reflections	10480
<I/σ(I)>	18.6
Completeness [%]	95.2	93.2
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5		PEG3400, Tris, sodium acetate, nickel chloride, pH 8.5, VAPOR DIFFUSION