3BQ5

Crystal Structure of T. maritima Cobalamin-Independent Methionine Synthase complexed with Zn2+ and Homocysteine (Monoclinic)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-21
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	73.229, 107.164, 107.667
Unit cell angles	90.00, 108.96, 90.00

Refinement procedure

Resolution	47.400 - 2.000
R-factor	0.218
Rwork	0.217
R-free	0.24030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t7l
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	EPMR
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.400	2.120
High resolution limit [Å]	2.000	2.000
Number of reflections	104398
<I/σ(I)>	15.6	4.4
Completeness [%]	99.8	100
Redundancy	4.8	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR BATCH, UNDER OIL	8.5	295	19.5% PEG 3350, 0.2M magnesium acetate, 2.5% ethylene glycol, 0.1M Tris-HCl, pH 8.5, VAPOR BATCH, UNDER OIL, temperature 295K