3BP8

Crystal structure of Mlc/EIIB complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6B
Synchrotron site	PAL/PLS
Beamline	6B
Temperature [K]	100
Collection date	2005-12-11
Spacegroup name	C 1 2 1
Unit cell lengths	201.427, 55.424, 82.460
Unit cell angles	90.00, 95.29, 90.00

Refinement procedure

Resolution	20.000 - 2.850
R-factor	0.228
Rwork	0.228
R-free	0.30100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1z6r 3bp3
RMSD bond length	0.009
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.850
Number of reflections	20356
Completeness [%]	92.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5		6% PEG 6K, 0.1M MgCl2, 0.1M sodium acetate, pH5.5