3BOR

Crystal structure of the DEADc domain of human translation initiation factor 4A-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-12-06
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97242
Spacegroup name	P 21 21 2
Unit cell lengths	58.093, 80.102, 42.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.850
R-factor	0.187
Rwork	0.185
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2g9n
RMSD bond length	0.015
RMSD bond angle	1.344
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (refmac_5.3.0037)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.920
High resolution limit [Å]	1.850	3.980	1.850
Rmerge	0.133	0.068	0.656
Number of reflections	17895
<I/σ(I)>	6.6
Completeness [%]	100.0	99.8	99.9
Redundancy	11.4	10.9	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.3	291	25% PEG 3350, 0.1M Bis-Tris, 0.2M Ammonium acetate. Chymotrypsin was added to the crystallization sample at a molar ratio of approx. 1:100, pH 7.3, VAPOR DIFFUSION, temperature 291K