3BOQ
Crystal structure of MarR family transcriptional regulator from Silicibacter pomeroyi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 61 |
| Unit cell lengths | 110.567, 110.567, 68.951 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.960 - 2.390 |
| R-factor | 0.20438 |
| Rwork | 0.203 |
| R-free | 0.23296 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.456 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.111 | 0.455 |
| Number of reflections | 18667 | |
| <I/σ(I)> | 17.52 | 4.98 |
| Completeness [%] | 97.9 | 100 |
| Redundancy | 8.4 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 3.5 M Sodium formate pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
